Analysis of The Accuracy of Temperature Sensors at The Calibrator Incubator Laboratory are equipped with data storage base on Internet of Thing

  • Candra Prastyadi Department of Medical Electronics Technology, Poltekkes Kemenkes Surabaya, Indonesia
  • Bambang Guruh Irianto Department of Medical Electronics Technology, Poltekkes Kemenkes Surabaya, Indonesia
  • Her Gumiwang Ariswati Department of Medical Electronics Technology, Poltekkes Kemenkes Surabaya, Indonesia
  • Dyah Titisari Department of Medical Electronics Technology, Poltekkes Kemenkes Surabaya, Indonesia
  • Steyve Nyatte University of Douala Cameroon, Cameroon
  • Shubhrojit Misra Department of Electronics and Telecommunication Engineering, Jadavpur University, India
Keywords: Incubator laboratory, Thermocouple, Calibration, IoT, SD card, MAX 6675


A laboratory incubator is a tool used to incubate or incubate a breed.  Incubators provide optimum temperature conditions for microorganisms to grow. The incubator has a temperature regulator so that the temperature can be adjusted according to the breed to be incarnated. Incubators utilize hot-dry like ovens. The purpose of this study is to conduct testing and analyze the accuracy of thermocouple sensors with incubator media in laboratory incubator calibrator tools. The contribution of the research is to know the level of accuracy of the sustainable sensor for sensing the temperature in the lab incubator. The main Design consists of 8 MAX 6675 Standards, 8 Thermocouple type K, Arduino Mega, and SD Card Standards. The temperature not in the incubator device is measured by a Type K thermocouple sensor. Thermocouple sensor numbers 8 channels that measure the temperature at each incubator camber point.   The temperature will be stored on the SD card to analyze the data and the data can be processed into the form of a graphic. Benchmarking is done using a data logger temperature tool.  This is done to make the Design results are under the standards of the Standard.  After comparing with the Standard get the largest error value is 3.98%, at channel T6 temperature 35 °C with ordinary incubator media and the smallest error in ordinary incubator media point T6 temperature 37 ° C which is 0.06 % and in fan incubator temperature 35 C has the largest error which is 2.98 % and the smallest error 0.86%. The conclusion of this study is that the design can work well in measuring the temperature of the incubator, as well as the system for storing readings using the SD card Design and sending data using the internet network can work well.


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M. K. Zarkani, “Design and implementation of Laboratory incubator,” Ministry of Higher Education & Scientific Research University of Kerbala, Republic of Iraq, 2020.

S. M. Lawal, M. Umar, and I. Muhammad, “Design and Performance Evaluation of an Automatic Temperature Control System in an Incubator,” Int. J. Appl. Electron. Phys. Robot., vol. 2, no. 1, pp. 8–12, 2017, doi: 10.7575/aiac.ijaepr.v.2n.1p.8.

A. Chauhan and T. Jindal, “Equipments and Instruments for Microbiological Laboratories BT - Microbiological Methods for Environment, Food and Pharmaceutical Analysis,” A. Chauhan and T. Jindal, Eds. Cham: Springer International Publishing, 2020, pp. 73–85.

A. Schertenleib, J. Sigrist, M. Friedrich, C. Ebi, F. Hammes, and S. Marks, “Construction of a Low-cost Mobile Incubator for Field and Laboratory Use,” J. Vis. Exp., Mar. 2019, doi: 10.3791/58443.

V. Thavaraj, B. Vashishth, O. S. Sastry, A. K. Kapil, and N. Kapoor, “Solar Powered Portable Culture Incubator,” Ann Pediatr Child Heal., vol. 3, no. 4, pp. 1–5, 2015.

D. Titisari and T. Kumar, “Nine Channels Temperature Data Logger Design for Dry Sterilizer Calibration,” vol. 15, no. 2, pp. 88–95, 2022.

B. T. Heligman et al., “The design and usage of a portable incubator for inexpensive in-field water analysis,” J. Humanit. Eng., vol. 6, no. 2, 2019, doi: 10.36479/jhe.v6i2.127.

U. Sarma and P. K. Boruah, “Design and development of a high precision thermocouple based smart industrial thermometer with on line linearisation and data logging feature,” Meas. J. Int. Meas. Confed., vol. 43, no. 10, pp. 1589–1594, 2010, doi: 10.1016/j.measurement.2010.09.003.

C. Bernardes, R. Bernardes, C. Zimmer, and C. C. Dorea, “A Simple Off-Grid Incubator for Microbiological Water Quality Analysis,” Water , vol. 12, no. 1. 2020, doi: 10.3390/w12010240.

S. F. Hussin and Z. Saari, “Portable Incubator For E.coli and Coliform Bacterial GrowthUsing IoT,” Adv. Comput. Intell. Syst., vol. 2, no. 1, pp. 1–9, Nov. 2020.

C. Gutierrez, A. Somoskovi, K. Natarajan, and D. Bell, “Need for better adherence to optimal incubation temperature for quality laboratory diagnostics and antibiotic resistance monitoring,” Afr. J. Lab. Med., vol. 7, no. 2, pp. 1–2, 2018, doi: 10.4102/ajlm.v7i2.789.

E. Clasen, K. Land, and T. Joubert, “Micro-incubator for bacterial biosensing applications,” in Fourth Conference on Sensors, MEMS, and Electro-Optic System, Feb. 2017, p. 100360G, doi: 10.1117/12.2245443.

J. Wight, M.-P. Varin, G. Robertson, Y. Huot, and A. Lang, “Microbiology in the Field: Construction and Validation of a Portable Incubator for Real-Time Quantification of Coliforms and Other Bacteria,” Front. Public Heal., vol. 8, p. 607997, Nov. 2020, doi: 10.3389/fpubh.2020.607997.

Y. Kusumawardani, E. Dian Setioningsih, and D. Titisari, “Water Bath Calibration Device with Data Storage Using Six Thermocouple Sensor,” J. Electron. Electromed. Eng. Med. Informatics, vol. 2, no. 2, pp. 40–47, Jul. 2020, doi: 10.35882/jeeemi.v2i2.2.

S. N. Syayakti, E. D. Setioningsih, and S. Sumber, “4 Channel Sterilizer Calibrator,” Indones. J. Electron. Electromed. Eng. Med. informatics, vol. 1, no. 2, pp. 65–70, Feb. 2020, doi: 10.35882/ijeeemi.v1i2.4.

M. Rofi’i, S. Syaifudin, D. Titisari, and B. Utomo, “Waterbath Calibrator with Nine Channels Sensor,” Indones. J. Electron. Electromed. Eng. Med. informatics, vol. 1, no. 1, pp. 1–6, Aug. 2019, doi: 10.35882/ijeeemi.v1i1.1.

B. L. Valdes-mora and P. Hardt-english, “Validation of a Laboratory Incubator Using Wireless and Cabled Datalogger,” J. Valid. Technol., vol. 8, no. 2, pp. 162–173, 2017.

IEEE SA, “IEEE Standards Activities in the Internet of Things ( IoT ) Overview,” no. November, pp. 1–4, 2018, [Online]. Available:

F. Puspasari, T. P. Satya, U. Y. Oktiawati, I. Fahrurrozi, and H. Prisyanti, “Accuracy Analysis of Arduino-based DHT22 sensor system against a Standard Thermohygrometer,” J. Phys. Appl., vol. 16, no. 1, p. 40, 2020, doi: 10.12962/j24604682.v16i1.5776.

D. Saepul Ramdan and M. Naufal Wijaksana, “Cold Storage Temperature Monitoring System Using Arduino-Based Data Logger and Visual Basic,” Sci. J. Informatics Manag. Comput., vol. 1, no. 3, pp. 107–112, 2017, doi: 10.32485/kopertip.v1i03.30.

Rizkiyatussani, Her Gumiwang Ariswati, and Syaifudin, “Five Channel Temperature Calibrator Using Thermocouple Sensors Equipped With Data Storage,” J. Electron. Electromed. Eng. Med. Informatics, vol. 1, no. 1, pp. 1–5, 2019, doi: 10.35882/jeeemi.v1i1.1.

A. Nur et al., “Development Of Application Programming Interface ( Api ) For Amikom Purwokerto Handsanitizer ( Ampuh ) Data Logger Pembangunan Application Programming Interface ( Api ) Untuk Visualisasi Data Logger Amikom Purwokerto Hand Sanitizer,”, Jurnal Teknik Informatika, vol. 3, no. 3, pp. 791–796, 2022.

A. Z. Febriyanti, P. C. Nugraha, and Syaifudin, “Temperature Calibrator Using Thermocouple Based on Microcontroller,” Indones. J. Electron. Electromed. Eng. Med. Informatics, vol. 2, no. 1, pp. 13–20, 2020, doi: 10.35882/ijeeemi.v2i1.3.

D. Singh, P. Kumar, and S. C. Prasad, “Calibration of thermocouples for low temperature applications,” in 2016 International Conference on Recent Advances and Innovations in Engineering (ICRAIE), 2016, pp. 1–4, doi: 10.1109/ICRAIE.2016.7939485.

Y. A. Abdelaziz, “Low Cost Humidity / Temperature Calibration System,” J. Sci. Eng. Res., vol. 4, no. 10, pp. 305–311, 2017.

O. R. Yunita, D. Titisari, and T. Hamzah, “Temperature Calibrator with Thermocouple Equipped with Graphic Display,” vol. 13, no. 1, pp. 32–42, 2020, doi: 10.35882/teknokes.v13i1.5.

Yunidar, Alfisyahrin, and Y. Rahmad, “Performance Analysis of NTC and LM35 Temperature Sensors in AVR ATmega 16 Microcontroller-Based Room Temperature Detection System,” J. Amplif., pp. 38–42, 2013.

Y. A. K. Utama, “Quality Comparison Between Temperature Sensors Using Arduino Pro Mini,” e-Jurnal Nar., vol. 2, no. 2, pp. 145–150, 2016.

A. Kus, Y. Isik, M. Cemal Cakir, S. Coşkun, and K. Özdemir, “Thermocouple and infrared sensor-based measurement of temperature distribution in metal cutting,” Sensors (Switzerland), vol. 15, no. 1, pp. 1274–1291, 2015, doi: 10.3390/s150101274.

E. Gavignet and F. Lanzetta, “Dynamic operation of a micro-thermocouple sensor as a vacuum gauge,” Vacuum, vol. 100, pp. 18–21, 2014, doi: 10.1016/j.vacuum.2013.07.043.

Syarifatul Ainiyah, D. H. Andayani, A. Pundji, and M. Shaib, “Development of Incubator Analyzer Based on Computer with Temperature And Humidity Parameters,” J. Electron. Electromed. Eng. Med. Informatics, vol. 2, no. 2, pp. 48–57, Jul. 2020, doi: 10.35882/jeeemi.v2i2.3.

S. P. Nalavade, A. D. Patange, C. L. Prabhune, S. S. Mulik, and M. S. Shewale, “Development of 12 Channel Temperature Acquisition System for Heat Exchanger Using MAX6675 and Arduino Interface,” 2019, pp. 119–125.

H. H. Shaker, A. A. Saleh, A. H. Ali, and M. A. Elaziz, “Self-calibrating enabled low cost, two channel type K thermocouple interface for microcontrollers,” in 2016 28th International Conference on Microelectronics (ICM), 2016, pp. 309–312, doi: 10.1109/ICM.2016.7847877.

S. Saha and A. Majumdar, “Data centre temperature monitoring with ESP8266 based Wireless Sensor Network and cloud based dashboard with real time alert system,” in 2017 Devices for Integrated Circuit (DevIC), 2017, pp. 307–310, doi: 10.1109/DEVIC.2017.8073958.

W. G. Shun, W. M. W. Muda, W. H. W. Hassan, and A. Z. Annuar, “Wireless Sensor Network for Temperature and Humidity Monitoring Systems Based on NodeMCU ESP8266,” 2020, pp. 262–273.

How to Cite
C. Prastyadi, B. G. Irianto, H. G. Ariswati, D. Titisari, S. Nyatte, and S. Misra, “Analysis of The Accuracy of Temperature Sensors at The Calibrator Incubator Laboratory are equipped with data storage base on Internet of Thing”, ijeeemi, vol. 4, no. 4, pp. 160-167, Nov. 2022.
Research Article